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Path C: Reconstitution (Mixing) Step-by-Step Guide

warning

Meticulous sterile technique is critical. Losing sterility at any point can introduce bacteria and cause serious infection.

  1. Prepare:

    • Wash hands with soap and water for at least 20 seconds, then dry.
    • Set up a clean, flat, uncluttered surface. Wipe it with household disinfectant and let it dry.
    • Lay out all supplies: peptide vial, BAC-water vial, mixing syringe and needle, alcohol swabs, and a sharps container. See Supplies Needed.
    • If either vial was refrigerated, let it reach room temperature before you begin. This prevents condensation on the glass.

  2. Clean vial tops:

    • Snap off the plastic caps from both vials to expose the rubber stoppers.
    • Using a new alcohol swab for each vial, scrub the stopper for at least 10 seconds.
    • Let the alcohol evaporate. Do not touch the stoppers after cleaning.

  3. Calculate and draw BAC water:

    • Decide how much BAC water is needed. Use the amount of peptide in the vial and your target final concentration. See Dosage Calculation.
      Example: A 10 mg vial mixed to 5 mg/mL needs 2 mL of BAC water.
    • Attach a sterile mixing needle to a sterile 3 mL or 5 mL syringe. Remove the cap without touching the needle.
    • Pull air into the syringe equal to the volume of BAC water you will withdraw.
    • Pierce the BAC-water stopper and inject the air. This equalises pressure.
    • Invert the BAC-water vial and slowly draw the measured volume into the syringe. Remove the needle from the vial.

  4. Add BAC water to the peptide vial:

    • Insert the needle through the centre of the peptide-vial stopper.
info

Crucial technique: Keep the needle bevel against the inside glass wall. Slowly depress the plunger so the liquid streams down the wall rather than splashing directly on the powder cake. This reduces foaming and protects the peptide.

  1. Mix the solution:
    • Withdraw the needle and place the used syringe and needle into the sharps container immediately.
    • Hold the peptide vial upright. Gently swirl in small circles or roll it between your palms until the powder dissolves and the solution looks clear. This can take a few minutes.
danger

Do not shake. Vigorous shaking creates foam and can denature the peptide.

  1. Inspect the solution:

    • Hold the vial up to a light. The solution must be crystal-clear, with no particles, cloudiness, or colour change.
    • If any particles remain or the liquid looks cloudy, discard the vial safely. It may be contaminated or inadequately dissolved.

  2. Label the reconstituted vial:

    • Write on tape or the vial label:
      • Date and time of reconstitution
      • Final concentration (for example, “5 mg/mL” or “5000 mcg/mL”)
      • Calculated expiration date (commonly 28 days in a refrigerator with BAC water. See Storage).
      • Your initials (useful if multiple people have supplies in the same fridge).

The peptide is now ready for Dosage Calculation followed by Drawing & Injecting.